A reaction monitored by guaiacol dye coupled with oxygen rep
Plant peroxidase is used in the process of lignin production (a polymer found in plant walls) and catalyses the breakdown of hydrogen peroxide in to oxygen. The release of oxygen can be measure by the oxidation of guaiacol in solution with peroxidase and its substrate hydrogen peroxide. An inhibitor, hydroxylamine, was added in different amounts (0.3, 0.5, 1.0,1.5, and 3.0mL) to the solution and enzyme activity was measured.The main function of an enzyme is to lower the activation energy of a biological reaction without itself being absorbed into the reaction (Thomas A. Scott, 1996). An enzyme is a protein made up of one or more polypeptide strands which can individually, or as a complex unit, take on a three dimensional shape (Campbell and Reece, 2002). These individual protein molecules mould to form an active site and other important structures that allow the enzyme to effectively catalyze reactions. Many variables can manipulate the effectiveness of an enzyme. One of which is an enzyme inhibitor. These are compounds or agents that combine with an enzyme in such a manner as to prevent the normal substrate-enzyme combination and the catalytic reaction (Campbell and Reece, 2002). There are two types o
ID Contents Buffer Hydrogen peroxide Dye (mL) Extract (mL)
Some topics in this essay:
Dii Eii,
Campbell Reece,
Cii Test,
Methods Materials,
Thomas Scott,
Abstract Plant,
Cii Concentrated,
Singleton Sainsbury,
Ei Eii,
Buffer Hydrogen,
hydrogen peroxide,
test tubes,
active site,
reaction rate,
inhibitor added,
10 0,
scott 1996,
0 20,
20 10 0,
20 10,
peroxide indicator dye,
tubes labeled,
test tubes labeled,
indicator dye 0,
0 20 10,
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Approximate Word count = 1439
Approximate Pages = 6 (250 words per page double spaced)
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