Effect of Regenerated Roots and Shoots

And Shoots on Nicotine production in tobacco

Tissue culture.” Drug Information Journal

Ambbler; July-Sept. 1998 Vol 32 Issue 3

The article “Effect of Regenerated Roots and Shoots on Nicotine Production I tobacco Tissue Culture of J. Saunders fully follows the IMRD structure. The article, as it appears in the Drug Information Journal (32(1998):609), is divided into four general sections. The general sections are: introduction, materials and methods, results and discussions, and references. The purpose of this paper is to resolve what relationship the different organs of the tobacco plant might have in the production of nicotine. From this information, the author concludes that there is an active turnover mechanism operating in shoots of tobacco culture, which requires a prodigious synthesis of nicotine in the plant to maintain high levels of nicotine found in tobacco issue.

The introduction of this article is consistent with the goals settled by the IMRD Introduction section. In the first paragraph, the author defines the use of tissue culture that their research focuses on and the facts that influences the variability of chemical constitut

The references of the author are possible to be articles also. It did not mention the publishers of the sources. It stated the authors, name of the articles, year released, volume number, and page numbers from the journals indicated. It did follow the number sequence citation throughout the article. All the sources are relevant to the methods and arguments made by the author.

The Results and Discussions became more scientific and specific. The first few paragraphs recall the method and data results of the experiment. It clearly stated the goal of the discussion in finding first the roles of the components of the plants namely the roots and the shoots. They have grown callus tissue on different concentrations of kinetin with indole acetic acid (IAA). The article clearly stated that the extracted nicotine from the root, shoot and callus tissue was spectacularly high when the callus rejuvenated roots. The rejuvenation of shoots in the rooted callus reduces the nicotine in the root and callus according to the article. And it tells that the increased in the nicotine content can be explained by the accumulation of the nicotine in the shoot and callus tissue caused by the rejuvenation of the roots in spite of the reduction of nicotine in the rejuvenation of shoots in the rooted callus.

The research also examined also the amount of nicotine concentration in regenerating shoot tissue with and without regenerated roots. This further explained the accumulation of the nicotine of the shoots. It has also been found out that there were some compounds prohibited to the root tissue as the nicotine were absorbed by the callus.

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Some topics in this essay:
Results Discussions, Results Discussion, IMRD Introduction, Information Journal, Callus Cultures, Chemicals Section, Ambbler July-Sept, Plant Material, Culture Saunders, Shoots Nicotine, callus tissue, shoot tissue, nicotine roots callus, levels nicotine, tobacco tissue, roots callus, nicotine content, extraction quantitation, nicotine roots, tissue culture, growth conditions, extraction quantitation nicotine, growth conditions callus, conditions callus cultures, drug information journal,