pH Affect the Rate of Enzyme Reactions

Enzymes are the molecules necessary for all the chemical reactions that take place in life. They will either build up molecules or break them down. Enzymes are catalysts, meaning they speed up the rate of reactions by lower the temperature at which the reaction takes place while remaining unaltered. The enzyme activity level may be affected if a reaction happens at too high or low of a temperature, too acidic or basic an environment, or too high a concentration. Laboratory 7 is to show the affects that concentration, temperature, and pH have in these reactions.

1% Sucrose (1g/99ml of distilled water) Buffers (pH 1.0, 4.4, 9.2, 13.0) Sucrase (1 packet yeast to 500ml distilled water) Ice Bath and Warm Water Bath

Test Tube Rack Test Tubes Pipette Beaker

Test Tube Clamps Benedict’s Solution Thermometer Distilled Water

C. Our final experiment tested the effects of ph on enzyme activity. In this experiment we first added 1.0ml of sucrase to each of our 4 test tubes and then their designated buffer. For test tube one 4.0ml of pH 1.0 was used, tube 2 used 4.0ml of pH 4.4, tube 3 used 4.0ml of pH 9.2, and tube 4 used pH 13.0. They were all mixed well and let to sit for 10 minutes at room temperature. Then 2.0ml of sucrose was added to each test tube and let stand for an additional 5 minutes. 3.0ml of Benedict’s solution was then added to each tube and placed in boiling water for 5 minutes to show the reaction.

B. Our thinking for experiment 2 was that the higher the temperature the faster or better the reaction to a point. Our hypothesis was proven correct. The best reactions happened at temperatures 30 and 60 degrees Celsius. 0 degrees Celsius was too cold causing the reaction to be very slow and 100 degrees was too high causing no reactions most likely due to the enzyme being denatured.

Some topics in this essay:
III Procedure, Results Page, Introduction Enzymes, II Materials, test tube, degrees celsius, benedict’s solution, ph 44, test tubes, benedict’s solution added, Reactions Page, Concentration Temperature, minutes 30ml benedict’s, 30ml benedict’s solution, minutes 30ml, boiling water, 30ml benedict’s, solution added, experiment tested effects, 10 minutes, Test Tube, ph 44 buffer,