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Electrophoresis

Electrophoresis is the process by which charged particles suspended in a solution may be separated through the application of an electric current. Electrophoresis was developed by Arne Tiselius. The process is used in medicine and biochemistry (science concerned with the organic and physical chemistry) to separate, identify, and measure the amount of various proteins. Electrophoresis is an analytical method frequently used in molecular biology (study of life processes) and medicine. It is applied for the separation and characterization of proteins, nucleic acids, and sub cellular-sized particles like viruses (parasite with a noncellular structure composed of mainly nucleic acid within a protein coat) and small organelles. Nucleic acid is an organic substance that plays a role in the storage and replication of hereditary information. Electrophoresis’ principle is that the charged particles of a sample migrate in an applied electrical field. In conducted in solution (homogeneous mixture of two or more substances), samples are separated according to their surface net charge density (ratio of mass of a substance to its volume). The most frequent applications, however, use gels (polyacryamide, agarose) as a support medium. Pr


Electrophoresis will continue to be an important process for scientists to use for many years. The fields of genetics and medicine will be able to use the information to study the structure of proteins from the interaction of several genes. A gene is a discrete unit of heredity information that specifies a protein. Genes have the code for chemical information necessary for the creation of a specific enzyme or protein. This type of information can be helpful to many people and improve our health care.

Electrophoresis is used in many different areas of science. This technology is demonstrated in the following areas: Electrophoresis of Protein, Electrophoresis of DNA, Gel Electrophoresis, and Polyacrylamide Electrophoresis. These methods of electrophoresis are unique but all lead to the goal of obtaining the specific identity, measurement, and separation of the substance being tested.

Electrophoresis is widely used to identify and measure the albumin, globulin, and fibrinogen fractions present in the blood. Albumin is a heat coagulating (clotting) protein, globulin is spherical proteins, and fibrinogen is a protein that causes plasma to gel. A large variety of instruments using this general principle are available. In research and industry, a technique called curtain electrophoresis is used in which the test solution flows down the edge of a horizontal covering. An electric current flowing across the covering carries the various fractions horizontally as they descend, depositing each in a separate collecting tube at the bottom edge.

Gel electrophoresis is a method used to separate biomolecules. The basic idea is that a solid support (the gel) is cast as a thin slab, either on a single plate (called agarose) or between two plates (called polyacrylamide). The slab is placed between electrode compartments with the polarity of electrodes depending on the material being separated. An electrode is the conductor through which electricity flows. A small sample of the material being separated is placed in a perform well or notch at the top of the gel, and the electric current I applied. Usually glycerol (colorless, odorless, alcohol)or other high-density material is added to the sample, often with a tracking dye, to ensure that the material stays in the well until drawn into the gel itself. This is required because polyacrylamide an

Some topics in this essay:
Polyacrylamide Electrophoresis, Arne Tiselius, Viral DNA, United Sates, UV Gel, , gel electrophoresis, amino acid, electric current, amino acids, protein electrophoresis, electrical field, types proteins called, acid organic, net charge, electrophoresis protein electrophoresis, gel fragments, separate identify,

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Approximate Word count = 1595
Approximate Pages = 6 (250 words per page double spaced)


  

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