cells
Basically, expts. # 9, 10 & 11 focused on cytoskeleton and cell motility. Expt. 9 required us to detach glycerinated rabbit psoas muscle into thin fiber bundles that when given ATP resumed contraction; moreover, demonstrating muscle fiber protein composition by means of SDS-PAGE was instigated. Then for immunoblotting our instructor began transferring of proteins from gel to nitrocellulose membrane. Finally, staining glycerinated muscle fibers with fluorescent nuclear dye ¡§DAPI¡¨ was done. Expt. 10 involved demonstrating the blotting technique which involved electrophoretically separating proteins that got transferred from gel to a nitrocellulose membrane filter. Eventually attempt to identify at least one of the separated proteins by the use of an antibody was made (actin). In Expt. 11 we had to apply technique of indirect immunoflurescence to demonstrate the location of microtubules in CHO and sea urchin sperm cells. Then staining of nucleic acid in CHO and sea urchin sperm cells with fluorescent dye DAPI was initiated. As a final point, we had to demonstrate the location of actin in glycerinated muscle fibers with the fluorescent probe ¡§Rhodamine Phalloidin.¡¨
Myokinase also produce ATP. ADP + ADP „²------------„³ ATP + AMP. Indirect Method: Rabbit immunoglobulins ----------„³ obtain ----------„³ Based on my results, as the concentration of ATP increases, so does the contraction increases as well. There were also noticeable changes in the length of muscle when ADP was added. The same result was observed as with ATP; as the concentration of ADP increases so does the contraction. Muscle cells have enzyme which not only breaks down ATP to ADP (creatin), but can also make ATP from ADP by using phosphokinase.
Some topics in this essay:
Motility II,
INTRODUCTION Basically,
Method Rabbit,
III Polyclonal,
AMP ATP,
IV DISCUSSION,
Phalloidin DAPI,
MATERIALS Expt#,
ATP ADP,
primary antibody,
Direct Method,
secondary antibody,
non-specific binding,
cytoskeleton cell motility,
cell motility,
atp adp,
glycerinated muscle,
control strip,
thin filaments,
cytoskeleton cell,
urchin sperm cells,
sea urchin sperm,
sea urchin,
gel nitrocellulose membrane,
thick thin filaments,
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Approximate Word count = 1260
Approximate Pages = 5 (250 words per page double spaced)
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