The purpose of this lab experiment was to see if a person could use a plasmid (R plasmid) vector containing certain fragments of foreign DNA for ampicillin resistance to be used to transform E. coli (Escherichia coli) cells to give the E. coli cells ampicillin resistance. Ampicillin resistance is needed in order for the E. coli to have the ability to survive in an ampicillin environment. Plasmid is a small ring of DNA that carries accessory genes separate from those of bacterial chromosome. Ampicillin is an antibiotic that is derived from penicillin that prevents bacterial growth by interfering with cell wall synthesis. E. coli is a commensal bacterium inhabiting the human colon that is widely used in biology, both as a simple model of cell biochemical function and as a host for molecular cloning experiments. In nature genes can be transferred between bacteria in different ways like transformation, conjugation, and transduction. One-way is bacterial transformation, it is when transfer of genetic information into a cell by the direct uptake of the DNA. Then the DNA is used to transform the cells from the original DNA to take a certain trait. How ever these bacteria can take up DNA only during the period at the end of logarit
6) Next take the tubes out of the waterbath and right away put them on ice for two minutes. (This part is critical to get right if not the plasmid would not of enter into he cells.)
One could hypothesize that plasmid would be able to successfully be incorporated in the E. coli cell. One would be able to see this by both agar plates without ampicillin and also the agar plate with ampicillin and plasmid would have bacteria growth. However the agar plate with ampicillin, but no plasmid will have no bacteria growth. One could hypothesize this because if the plasmid were incorporated into the E. coli cell correctly the E. coli would have ampicillin resistance.
3) Then pass the transfer loop over the flame of the bunsen burner to clean it and repeat procedure 2 for the + tube.
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