C-Fern Lab

In this lab we took a green F1 diploid sporophyte population and allowed meiosis to occur to produce G2 haplolid gametophytes. Of the two cultures, Hybrid A gametophytes were produced in equal numbers of both mutants, while Hybrid B produced significantly more pale. These populations were allowed to fertilize randomly to produce a diploid F2 sporophyte population. Because of this diploidization, I hypothesized that there would be many more green sporophytes than pale ones in Hybrid A, since green is dominant (Lab Manual 2.2). I made this assumption believing that Hybrid A would follow the expected Mendelian ratio of a monohybrid cross, which is 3:1 (Klug & Cummings 46). The results obtained for Hybrid A held true to this hypothesis. Hybrid B followed a different pattern of inheritance (Lab Manual 2.2). After I obtained the class results for the F1 and F2 populations I hypothesized that the pattern of inheritance for Hybrid B was due to epistasis of one of the alleles in a dihybrid cross. The results of Hybrid B held true to the Mendelian ratio for dihybrid crosses, 9:3:3:1 (Klug & Cummings 54), with one of the alleles exhibiting epistati

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