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Koch's Postulates

Koch created four guidelines to determine the causal agents of disease in humans, animals, and plants. Koch proved that a disease-causing agent could be transferred from one organism to another and create the same illness. Isolation of pure cultures and the introduction of the disease-causing agent to a healthy organism will transmit the disease and infect the inoculated organism. Koch’s four guidelines by which one must follow to transmit a disease from an infected organism to a healthy one are as followed:

1. The specific organism should be shown to be present in all cases of animals suffering from a specific disease but should not be found in healthy animals.

2. The specific microorganism should be isolated from the diseased animal and grown in pure culture on artificial laboratory media.

3. This freshly isolated microorganism, when inoculated into a healthy laboratory animal, should cause the same disease seen in the original animal.

4. The microorganism should be reisolated in pure culture from the experimental infection.

In this exercise, Penicillium was utilized, a common, safe, mold. Certain species of Penicillium will spoil fruits, vegetables, grains, and grasses.


17. Streak a Potato Dextrose Agar plate and incubate at room temperature for approximately 5 days.

Microscope views of the fungus appeared as sticks with budding hyphae. The blue-green mold had more hyphae than the white mold.

3. Discuss a scenario using a human disease and how Koch;s postulates can be used.

2. What is the purpose of the scrub brush in the wash cycle?

13.Make a slide of your inoculating organism.

Other species will ripen various chesses. Still, other species are used in the production of antibiotics. The species of Penicillium, italicum is provided for the lab because of its pronounced hyphae. Penicillium italicum, along with Penicillium digitatum attack citrus fruits post-harvest. In this experiment, the effect of Penicillium italicum on two types of citrus fruits and one non-citrus fruits were tested.

Koch’s Postulates not only provided a logical basis for concluding that a particular microorganism is the etiologic agent of a given disease, but also opened the door for an enormous enthusiastic search for the causes of infectious diseases.

Some topics in this essay:
Materials Methods, Introduction Koch, Dextrose Agar, Postulates Blood, Koch’s Postulates, Discussion Questions, Results Macro-, AIDS HIV, Micro- Microscope, Laboratory Review, citrus fruits, koch’s postulates, penicillium italicum, sterile applicator, disease found, sterile applicator stick, pure culture, applicator stick, flame tube recap, penicillium digitatum, species penicillium, growth evident, penicillium italicum penicillium, tube recap discard, italicum penicillium digitatum,

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Approximate Word count = 963
Approximate Pages = 4 (250 words per page double spaced)


  

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