Optimum Temperature And PH For Enzyme Alpha-amylase
The purpose of this lab was to determine the optimum pH and temperature for the reaction of the enzyme a-amylase with the substrate starch. I worked with an enzyme and a substrate. An enzyme increases the reaction rate and lowers the activation energy of an efficient reaction. Enzymes are composed of proteins, which are amino acid chains (Campbell et al, 1999). The enzyme that I used was a-amylase; an enzyme extracted from barley seeds but also found in the human body. The reactant in an enzymatic reaction is called the substrate. I used a starch solution as the substrate in this experiment. An enzymatic reaction occurs when the enzyme and substrate collide and form an enzyme-substrate complex (Vliet, 1996). This complex quickly breaks down leaving the enzyme and a product or products. In this reaction of a-amylase with starch the product is glucose. This is the same reaction that occurs in the germination of barley seeds. The barley plant embryo produces a-amylase and releases it into the endosperm. The enzyme then reacts with starch to create glucose and nourishes the seed (Vliet, 1996). Since the purpose of this lab was to observe changes in rate reaction, I used I2KI indicator that reacts with starc
After finishing all the experiments and plotting all the data, I evaluated the results of my experiment. In the introduction I hypothesized that at higher temperatures the reaction rate would be greater due to increased particle collision. From the data that I have this claim is relatively supported. There are two notable exceptions, when the temperature was 45 and 55 degrees C. These two rates do not coincide with the rest of my findings, leading me to believe that an error occurred while doing the experiment. This error could be from the fact that the temperature fluctuated with the interaction of room temperature with that of the water. Another possible source of error could be that my measurement of either the I2KI or the reaction flask fluid was not consistent or correct. Future considerations that I would experiment with are trying to zero in on a more precise optimum temperature by using smaller intervals around the approximate optimum that I found, and trying to find the temperature at which it is too hot for a-amylase react and denaturing occurs.
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Approximate Word count = 1129
Approximate Pages = 5 (250 words per page double spaced)
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