PART A: In the fist part of the lab, the catalase is mixed with substrate (hydrogen peroxide). The enzyme catalyzes the conversion of H2O2 to H2O and O2. Before all of the catalase is converted, sulfuric acid (H2SO4) stops the reaction. After the reaction is stopped the amount of H2O2 remaining is measured by the use of potassium permanganate (KMnO4) added in drops until the solution turns a reddish brown or pink color, the final reading on the titration tube is the amount of H2O2 that remains in the solution.

PART B: In order to determine the amount of H2O2 initially present in a 1.5% solution, 10mL of H2O2 is added to a beaker followed by 1 mL of H2O and 10 mL of H2SO4. after mixing thoroughly, 5mL of the solution is removed and assayed for the amount of H2O2 present (following the above procedure using KMnO4).

PART C: To determine the decomposition rate or H2O2 after using a purified catalase extract, place 10 mL of H2O2 and 1mL of catalase in a beaker and swirl for 10 seconds before adding 10 mL of H2SO4 to stop the reaction. For the 30 experiment, begin the

7. Cold-water drowning victims can survive prolonged periods under water because the cold water would slow the chemical reactions within the body as well as slow down the oxygen consumption, which in turn slows down processes like cellular respiration. For this reason, cooling tissues below normal temperatures (98.6 degrees Fahrenheit/ 37 degrees Celsius) can prevent injury from inadequate oxygen.

8. It is not necessary to have on enzyme molecule for every substrate molecule that needs to be catalyzed because the enzymes are re-usable once they finish working with one substrate molecule.

Some topics in this essay:
Amount H2O2, H2O O2, Activity KMnO4mL, Assay H2O2, , MATERIALS METHODS, H2SO4 KMnSO4, Amount KMnO4, amount h2o2, enzyme catalase, VARIABLE Temperature, 33 33, assay h2o2, final reading, 10 ml, 33 33 33, 10 ml h2so4, amount kmno4, reaction stopped, rate h2o2, initial reading, 60 120 180, assay h2o2 enzyme, h2o2 enzyme catalase,