beetroot membrane

effecyThe Effect Of Temperature On A Plasma Membrane

This experiment aims to determine what effect an increase in the surrounding temperature has on the plasma membrane of a typical plant cell structure.

An increase in temperature will damage and denature the plasma membrane and cause the cytoplasm and other substances contained within the membrane to leak out.

The purpose of a cell membrane is to control the transport of substances moving into and out of a cell. The membrane is an extremely thin layer (8 to 10 manometers (nm)) thick, which is partially permeable. It consists mostly of lipids and proteins. The lipids found in cell membranes belong to a class known as triglycerides, so called because they have one molecule of glycerol chemically linked to three molecules of fatty acids. The majority belong to one subgroup of triglycerides known as phospholipids.

Despite their many differences in appearance and function, all cells have a surrounding membrane (called the plasma membrane) en

Beetroot Plant Corers (various diameters) White tile Heat proof mat Bunsen burner Tripod Gauze Beaker (for water bath) Thermometer ColorimeterDistilled Water7 test tubes containing 10cm³ of waterTongsScalpelStop-clockTest tube rack

Before the experiment can start, the beetroot must first be prepared. To do this, we need the white tile and the corers. The same diameter corer must be used for all the pieces so keep the surface area of each beetroot piece fairly similar. To collect a cylinder of beetroot, simply push the corer into the vegetable and then withdraw it. The cylinder will remain inside the corer, so it must be pushed out with a corer of a smaller diameter. Once a few good, uniform cylinders have been collected, they must then be cut into 7 pieces of equal length. The beetroot was cut to 1cm. Because the beetroot has been cut some of the cell membranes had been broken, which means some anthocyanin will leak out. This must be completely washed off in order to maintain the reliability of the results.

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