... listed except 50ul Glycogen phosphorylase b solution was exchanged for enzyme dilution buffer. 50 uL of glucose-1-p dilutions were added to their labeled test tube and incubated for 5 minutes in a water bath at 37C. The reaction was stopped by working Stopping Solution and read in a spectrometer 30 minutes later. To test substrate dependence in the presence of 1-methylnicotinamide, the same setup of ... were created with all reagents listed above but Glycogen phosphorylase b solution exchanged for enzyme dilution buffer. 50ul of G-1-P solution was added to its labeled test tube and incub...

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• Grade Level: Undergraduate