... we are monitoring its dissociation from the SH3 domain with increasing competitor (2AQ) binding (non-competitive or competitive binding?). See end of document for graphs detailing binding of ligands to the SH3 domain. Experiment has two steps: 1) Synthesis of protein of interest by: Purchase of multiple (up to) 110 nucleotide DNA templates which can be placed tail-to tail with ~16 nucleotide overlapping sequences, to synthesize ... the whole domain of interest. PCR of the 'patchwork' DNA templates to produce a whole DNA template molecule for transcription/translation into required prote...

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