80 degrees the rate of increase in absorbance and therefore concentration of the red pigment released from the beetroot, actually stops increasing. The explanation for this is that the concentration gradient of the red pigment from the inside of the cells in the beetroot, compared to the distilled water outside containing red pigment, has been dramatically lowered. This gradient is eventually cancelled out, with the same amount of red pigment in the cell, compared to the outside of the cell. The reprucusions of this, is a stop in increase of the red pigment released from the beetroot at these high temperatures, hence meaning that the amount of red pigment released from the beetroot and therefore absorbance of the solution stops increasing. Hence the absorbance will stay at this constant rate, and will not rise or fall at all even if the temperature is changed. This is because no further pigment can pass from the inside of the beetroot cells to the distilled water outside, due to their no longer being a concentration gradient, hence meaning diffusion can no longer operate. .
Therefore, from the results that I have obtained I can make some extremely interesting, valid conclusions. Firstly I can conclude that at low temperatures, between 25 and 50 degrees celcius, that an increase in temperature does undoubtedly cause a slow increase in the amount of red pigment released from beetroot. A further increase in temperature between 50 and 70 degrees celcius, causes an even more rapid increase in the release of red pigment from the beetroot. However, over 70 degrees celcius, an increase in temperature has no further effect on the release of the red pigment from the beetroot. .
Evaluation .
On the whole I am delighted with the data obtained in this investigation, as the graph and line of best fit do undoubtedly portray an excellent trend, despite their being a few anomalies. The fact that I conducted three experiments at each individual temperature, and then took an average from these to plot the graph, means that some anomalies visible in my table of results are actually masked on the graph.
