1. One Step Growth Curve Of T4 Bacteriophage In E.coli
... 10-2, IC 10-4 and IC 10-6 respectively. The IC tubes were used to determine the total infectious centers in the ADS tube. Two top agar tubes were then inoculated with 300L of indication E. Coli culture each. One of these tubes received 50L of the 10-6 IC culture and ... at 37C. In addition one control plate was made by adding 300L of indicator culture to liquefied top agar. The top agar containing the E.coli was poured onto a base agar plate. This plate was incubated along with the 14 test plates for 48 hours at 37C. After 48 hours, ... is apparent that the rise phase of ...
- Word Count: 2348
- Approx Pages: 9
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- Grade Level: High School