... which would indicate gram-positive cocci since MacConkey inhibits gram-positive growth. The Catalase test was negative which indicates a Streptococcus sp. The Mannitol salt showed no growth and the Blood agar showed Beta Hemolysis. All of these reactions are properties of Streptococcus Pyogenes. Discussion: Streptococcus Pyogenes a Group A Streptococcus is a Gram-positive, non-motile, non-spore forming coccus ... fermentative and is a catalase-negative facultative anaerobe, and requires enriched medium containing blood in order to grow. Group A streptococci typically have a capsule and ex...

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• Grade Level: High School

... 10-2, IC 10-4 and IC 10-6 respectively. The IC tubes were used to determine the total infectious centers in the ADS tube. Two top agar tubes were then inoculated with 300L of indication E. Coli culture each. One of these tubes received 50L of the 10-6 IC culture and ... at 37C. In addition one control plate was made by adding 300L of indicator culture to liquefied top agar. The top agar containing the E.coli was poured onto a base agar plate. This plate was incubated along with the 14 test plates for 48 hours at 37C. After 48 hours, ... is apparent that the rise phase of ...

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• Has Bibliography
• Grade Level: High School

... reco! gnized and extensively studied bacteria that often get more attention during the winter is Streptococcus. There are different strains of strep. Depending on their ability of hemolysis of blood agar, strep can be subdivided into a, b and g hemolytic (1-pp143). There are many different laboratory tests that can be done to distinguish one strep from the other. Different treatments ... peptidoglycan. Peptidoglycan can retain the violet blue, and does not wash off with alcohol. It grows in short chains, containing several streptococci. It looks a lot like a string of beads. Blood agar...

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• Grade Level: High School

... were thoroughly wiped down with 70% alcohol, and a Bunsen burner was used to sterilize all equipment used. Milk Experiment: In 8 petri dishes, we poured agar to act as a medium to culture the bacteria. We then used 2 dishes each for control groups, fresh milk, 24 hour old milk, 4 day old ... . Crystal violet staining detects the presents of bacteria. Gram staining identifies volatile verses nonvolatile bacteria. E. coli Experiment: In 8 petri dishes, we again poured agar to act as a medium for the bacterial growth. We then smeared all 8 with the bacteria E. Coli. We kept 2 for control gro...

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• Has Bibliography
• Grade Level: High School

... the air (group 5, control group), respectively. The samples were put in 5 different petri dishes. Then, the petri dishes were placed in the same incubator. All other factors, such as the agar in the dishes, temperature, as well as sampling time, were kept constant. After two days, the result showed that the sample from the screen had more colonies than the samples ... that the sample collected from the keyboard and touchpad would have more bacteria colonies than the samples from the speaker and the screen. Materials & Methods Materials: 5 sterilized petri dishes with agar (bacterial foo...

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• Has Bibliography
• Grade Level: High School

... was streaked off of the bottom of a rock that was planted on the soil, using a sterile cotton swab. The environmental unknown was made pure by streaking colonies onto a trypticase soy agar (TSA) plate until all of the colonies were of uniform morphology. The first TSA plate was incubated at room temperature, 25ï‚°C, for 72 hours then refrigerated at 4ï&sbquo ... then sat a room temperature for 24-48 hours, until the growth was then checked. Once the unknown was pure a single large colony was selected and inoculated onto two tripticase soy agar slants with a screw cap tube for stora...

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• Has Bibliography
• Grade Level: High School

... . Independent Ultraviolet radiations (placed on four quadrant of petri dish, with exposure to each quadrant of 10's, 20's, 30's, 40's). Dependent Color change and survival of Serratia marcescens. Control Nutrient agar plate with streaks of Serratia marcescens without UV light. Results S.marcescens is a gram negative rod shaped bacteria, containing pigment prodigiosin. The control group after incubating at 25 degree Celsius, showed ... ...

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• Grade Level: High School

... and how the MRVP reactions are used to identify metabolic end products. Recognize the appearance of a positive citrate reaction. Materials: E.Coli, Enterobacter Aerogenes 2 tubes MRVP broth, MRVP reagent 2 Simmons citrate agar slants, inoculating loop and Bunsen burner. Procedure: Label tubes and use loop to aseptically inoculate bacteria to MRVP tubes. Do same for all organisms. Incubate the tubes at 37o. Remove ... ...

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• Grade Level: High School

... rods. I though I was going to have a difficult time trying to identify one species until I looked at the TSA plate. One of the bacteria gave off a blue-green pigment into the agar, this is only characteristic to one bacterium on the list of specific bacteria we could have. The other bacteria had a cream growth on the TSA plate but a red colony on ... ...

• Word Count: 765
• Approx Pages: 3
• Grade Level: High School