1. Water paper
... bubble in Durham tube. II. Material and Methods A. Presumptive Test: Inoculate three Lactose broth tube with 1 ml of water from each of the water sample A, B and C. Incubate at 37 degree C for 24-48 hours. Water sample turing yellow in color is positive for Acid and Bubble in Durham tube shows positive for Gas production in 24-48 hours positive presumptive test. B. Confirmed test includes ... Escherichia coli is confirmed positive. C. For completed test inoculate Lactose broth from isolated colony and incubate at 37 degree C for 24-48 hours. Acid indicated by water sample turning yello...
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