... that concentration, temperature, and pH have in these reactions. II. Materials 1% Sucrose (1g/99ml of distilled water) Buffers (pH 1.0, 4.4, 9.2, 13.0) Sucrase (1 packet yeast to 500ml distilled water) Ice Bath and Warm Water Bath Test Tube Rack Test Tubes Pipette Beaker Test Tube Clamps Benedict's Solution Thermometer Distilled Water Marker Hot Plate III. Procedure A. The first experiment tested the effects of concentration on ... of sucrose and pH 4.4 buffer but each were kept in designated temperatures. Test tube 1 was kept in at 0 degrees Celsius (an ice bath), tube 2 was kept at 30...

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• Approx Pages: 3
• Grade Level: High School

... tube will be placed in different temperature water baths. The first tube will be placed in an ice bath that should maintain a temperature of 30F. Another tube should be kept at a constant 70F water bath and the other tube should be placed in water bath at a temperature of 100F. There will be no substrate in these control tubes. After 20 minutes, a spectrophotometer can ... ...

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... any anomalies. Apparatus The apparatus I would use for this experiment is: - Test tube - Test Tube rack. - Hydrogen peroxide (H2O2 in different concentrations). - Stop watch. - Potato. - Beaker - Cork borer - Syringe. - Water bath Method To do this experiment I would use 15 one-millimetre thick potato disks by cutting them, once taking the potato out by using a cork borer. I would use these 15 disks ... in all the experiments because the catalase will not run out but the hydrogen peroxide will. I will use a water bath to keep the temperature the same as usually enzymes work better with ...

• Word Count: 871
• Approx Pages: 3
• Grade Level: High School

... compounds and are thus well suited as solvents for this reaction. Procedure: Rapidly weigh 12 g of KOH into 100-ml rb flask, add 30 ml dimethoxyethane, immediately cool in ice bath. Swirl for two minutes, the purge with nitrogen for two minutes. Quickly stopper flask and shake to dislodge KOH cake (much will remain undissolved). Grind 3.5 g iron(II) chloride tetrahydrate to ... ...

• Word Count: 793
• Approx Pages: 3
• Grade Level: High School