Decapitated coleoptile in 1ppm IAA showed the largest average growth at 11.6mm, while decapitated coleoptile in water showed no growth.
Material and Methods.
In the experiment, I had seven different groups that we were using to test the effect of IAA concentration on coleoptile growth. One of the seven treatment groups received water and was cut to 18mm long, with the apex still intact. All the other treatments had 3mm of the apex removed and was then cut to 15mm in length and had varying amounts and concentrations of IAA added to them. Each treatment group had ten coleoptiles in one petri dish. The groups were as assigned as followed: (control) intact coleoptile in water, decapitated coleoptile in water, decapitated coleoptile in .1ppm IAA, decapitated coleoptile in 1.0 ppm IAA, decapitated coleoptile in 10.0 ppm IAA, decapitated coleoptile in 100.0 ppm IAA, and decapitated coleoptile in 1000.0 ppm IAA. All groups of ten coleoptiles were stored in the dark for one week, then we pulled them out of storage and had each of the ten coleoptile recorded of their length in each treatment group. I then calculated the average total length, average growth, and standard deviation.
Results.
The best average growth was shown by treatment group (decapitated coleoptile in 1.0 ppm IAA), which was 11.6mm. The next best was treatment group (decapitated coleoptile in 10 ppm IAA), with growth of 8.6mm. Next was treatment group (intact coleoptile in water), with growth of 8.85mm. Decapitated coleoptile in .1 ppm IAA, grew 7.5mm. Decapitated coleoptile in 100.0 ppm IAA had an average growth of 4.9mm. Decapitated coleoptile in 1000.0 ppm IAA grew only .475mm and Decapitated coleoptile in water had no growth at all. Treatment groups that had a concentration of 1.0 ppm showed the highest average growth at 11.6mm. Coleoptiles in IAA at 1000.0 ppm showed the least average amount of growth at .475mm. All treatments that contained an amount of IAA resulted in an increase of growth compared to the negative control group.
