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Diffusion and Osmosis Lab

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             .
             Solutions.
             Ho: The weight of the deshelled eggs will not be affected by different sucrose.
             .
             Solutions.
             Expectations.
             For this lab I expected that the solutions with different amounts of sucrose would impact the weight of the naked eggs. I expected the eggs to change in size because the membrane protecting the yoke of the eggs is permeable and will allow water to flow through and out of it. I did expect for the deshelled eggs to be affected. The eggs have water inside the membrane to begin with so I expected them to lose more water and shrink in higher concentrations of the sucrose solutions. In lower concentrations of the sucrose solutions, I expected the eggs to absorb more water from the surroundings and become larger in size. .
             Methods and Materials.
             According to the directions in the laboratory packet necessary for the lab, my lab mates and I were required to measure the effects on the deshelled eggs from known sucrose solutions. We also were directed to analyze those measurements and determine of the concentration of an isotonic solution and specifically the unknown solution. These materials were needed for the lab:.
             Pencil .
             Paper.
             3 deshelled eggs.
             3 sets of paired incubation cups.
             1 triple beam balance .
             Sucrose solution (either 10%, 20%, 30%, 40%, or unknown).
             Distilled water.
             My lab mates and I were given the unknown sucrose solution to experiment with. After gathering all materials, we began putting the experiment together. Marking 3 sets of plastic cups A, B, and C allowed us to keep the three deshelled eggs separate. The first set of 3 labeled cups did not have holes in the bottom. Those cups held the unknown sucrose solution. The other sets of paired cups did have hole cut in the bottom to allow the solution to drain out. The cups with the holes are the cups that the deshelled eggs are placed in. We then poured our unknown solution into the three cups without holes to the correct amount. Using a triple beam balance, we weighed the cups with the holes in the bottom (in grams) so we could calculate the overall weight of the egg by subtraction.


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