We want to find out how enzymes affect chemical reactions in the production of ATP. ATP is an adenine-containing nucleoside triphosphate that releases free energy when its phosphate bonds are hydrolyzed. This energy is used to drive endergonic reactions in cells. In our experiment we will utilize ATP as an energy source to facilitate the conversion of luciferin to its oxidized form by luciferase. We will measure the nucleotide specificity of the luciferase enzyme and how it is influenced by pH changes. We will also measure how different reagents affect the luciferin and luciferase mixture, called lantern. The production of ATP in a biological system requires numerous biochemical reactions that are mediated by various enzymes. An enzyme is a protein serving as a catalyst, which is a chemical agent that changes the rate of a reaction with out being consumed by the reaction. These proteins have a specific three-dimensional shape that includes an active site that fits its specific substrate, which is simply what makes the enzyme work. In this experiment the objective is to use what we know about the release energy in the form of light from the ATP depending on the substrates added. Theoretically, when luciferin mixes with the oxygen in the air and it joins to the active site of luciferase, there should be no reaction because luciferase requires 2 substrates to activate and release energy. When ATP is added, however, it binds with luciferase and breaks off one phosphate group of ATP, creating adenosine diphosphate (ADP). This will release much energy in the form of light that will be visible in a dark room. The release of energy is due to the first law of thermodynamics, which states that energy cannot be created or destroyed, it can only take on different forms. Here, some of the energy will go to make oxidized luciferin, some will come off as heat, and most is released as light.
Implementations of things like libraries, computer labs, or scientific labs increase test scores in reading and mathematics (Severin, Eugenio). ... The only question is, which piece of infrastructure is best to implement: computer labs, libraries, or scientific labs? Methodology The three different pieces of infrastructure that we decided to examine were: computer labs, libraries, and scientific labs. ... There were three research reports that were examined to collect useful information. ... The report when on to describe how laptops resulted in higher test scores (Duarte et Al). ...
1.Introduction A. An experiment of measurement, in which we will measure the length from a persons back of the knee to their heel, then measure their height and plot the results on to a graph to show any relationship between the height and the length of the leg. B. The experiment being cond...
Lab Report for Chemical Reactions Experiment Objective: In this experiment students will attempt a number of different reactions. The students will confirm that a reaction did/did not occur by detailed observation, and predict the products of each reaction by performing basic tests. Hypoth...
Pre-Lab Part A: 1. ... Cell wall- protects the cell Plasma membrane- boundary between cell and environment Nucleolus- makes ribosomes Chloroplast- captures light Cytoplasm- clear gelatinous fluid Pre-Lab Part B: 1. ... We did make an error in the process of the lab. ...
LAB QUESTIONS 1. ... There are many small factors that could have contributed to error in this lab. ... EVALUATION This lab was a good lab to start the year off with. ... What this lab taught me about mass, volume, and density. ... This lab will prepare you for other labs cause it taught you the basic skills you need to know. ...
LAB: DETERMINING THE VALUE OF THE UNIVERSAL GAS CONSTANT, R. Objective: The objectives of this lab are to measure the volume, pressure and temperature of a sample of wet butane gas, C4H10 grams. ... Put on safety glasses and keep on until you complete the lab. 2. ... Obtain butane lighter from your instructor v/s NO NOT LIGHT AT ANY TIME DURING THE LAB. 6. ... As one partner holds the graduated cylinder (inverted), the other lab partner should hold the butane lighter under the water just below the opening of the graduated cylinder is equal the level outside when finished. 8. ...
For our Pineapple Bromelin Enzyme lab, our group had predicted that the gelatins that contained Fresh and Frozen pineapples would liquidity and the gelatins that contained Boiled and Canned pineapple would stay as solid like normal gelatins do. ... For our fresh pineapples we knew that it had to come out with liquid gelatin because it contained bromeliad enzyme that break down animal fat in gelatins but in our lab it came out to be solid. ... This lab has thought us many knowledge and furthermore it led us to a question that is there any temperature or any conditions that enzymes perform bette...
Teaching as My Career For the last twelve years or so, I have been going to school. Every single day I find myself behind a desk doing schoolwork. Day in and day out it is the same thing, come to school, do some work, leave school to go home, and when I get home, I do even more work. But this...