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Microsatellites In DNA

            
             The article "Agents of Evolution" addresses the topic of microsatellites in.
             The article explains that DNA contains satellite DNA. There are 3.
             types of satellites. The first is satellite DNA. These are tandomly repeated pieces of.
             DNA. The second is minisatellite DNA. These are tandomly repeated pieces of DNA of.
             at least 10 base pairs. The third is microsatellite DNA. These are tandem arrays of.
             mono- to tetranucleotides in DNA. Of the 3 billion nucleotide bases only 10% of them.
             comprise of genes. The rest are satellites and have no apparent purposes. .
             The findings consist of bacteria enhancements to human restrictions. The results.
             are based on microsatellite DNA. The article is relevant to genetics by talking about.
             mutated genes of bacteria because of microsatellites and diseases in humans caused by.
             these same microsatellites. It also explains why this is important to evolution. This.
             technique of finding these satellites can offer geneticists a new world to explore. These.
             satellites are ever changing and helpful in detection of some diseases.
             To obtain DNA and run the polymerase chain reaction one must first obtain DNA. .
             To do this one will swab the inside of the cheek for cells. This is then placed in a test.
             tube . 10 microliters of DNA is needed. 6 microliters of water must be added to the test.
             tube. Vortex the tube for 15 seconds. Incubate the tube at 65 C for 30 minutes. .
             Vortex the tube for 15 seconds. The primers are now added to the test-tube. 1.5.
             microliters of each primer is then added to the DNA. There are 3 primer sets, so 6.
             primers must be added. The temperature is then raised to 98 C. It must be incubated for.
             15 minutes. The 25 microliters redimix is now added and the polymerase chain reaction.
             takes place. The sample is denatured at 94 C for 20 minutes. This allows the DNA.
             strand to separate. The annealing step is next. Lower the temperature to 57 C for 45.
             seconds.


Essays Related to Microsatellites In DNA

1. Gene Therapy for Alzheimer's Disease

The causes of Alzheimer's are attributed to causes such as, the inheritance of specific gene, stress, DNA methylation in the brain and even lifestyle choices like smoking. ... This method included Exonic Real-Time quantitative PCR, Fluorescent microsatellite PCR and Illumina array. ... The Fluorescent microsatellite q-PCR, APP alleles were quantified by the gene typing of 2 microsatellites located within intron 1 of APP and 330 kb centromic from APP....

  • Word Count: 5609
  • Approx Pages: 22
  • Grade Level: Undergraduate

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