1. One Step Growth Curve Of T4 Bacteriophage In E.coli
... round of virus multiplication using a one step growth curve. This method is possible due to the synchronization of replication of phage in the sample (Flint, 2002). A one step growth curve experiment begins when bacteriophage are infected with a very large number of phage particles. The large ... , by dividing the PFU on each plate by the number of bacterium in the ADS the PFU per bacterium amount was calculated. Table 1 contains summarized results for these calculations. Sample Calculation for bacteria/mL ADS Bacterium concentration in ADS tube: C1V1=C2V2 (0.10mL)(2 x 10 8) = (0.200mL) x...
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