... which breaks down the hydrogen peroxide A Measuring cylinder - to collect the oxygen which forms once the hydrogen peroxide has decomposed. Water bath – this is a thermostatically controlled bath which enables the experiment to be carried out at precise temperatures. A bung with plastic tubing – this is used to ... connect the first boiling tube to the collecting bath. A stopwatch – this is to enable the experiment to be accurately timed The collision theory is based on the principle that for ... that is produced as the hydrogen peroxide decomposes. A second boiling tube will be f...

• Word Count: 1786
• Approx Pages: 7
• Grade Level: High School

... which breaks down the hydrogen peroxide A Measuring cylinder - to collect the oxygen which forms once the hydrogen peroxide has decomposed. Water bath – this is a thermostatically controlled bath which enables the experiment to be carried out at precise temperatures. A bung with plastic tubing – this is used to ... connect the first boiling tube to the collecting bath. A stopwatch – this is to enable the experiment to be accurately timed The collision theory is based on the principle that for ... that is produced as the hydrogen peroxide decomposes. A second boiling tube will be f...

• Word Count: 1867
• Approx Pages: 7
• Grade Level: High School

... listed except 50ul Glycogen phosphorylase b solution was exchanged for enzyme dilution buffer. 50 uL of glucose-1-p dilutions were added to their labeled test tube and incubated for 5 minutes in a water bath at 37C. The reaction was stopped by working Stopping Solution and read in a spectrometer 30 minutes later. To test substrate dependence in the presence of 1-methylnicotinamide, the same setup of ... were created with all reagents listed above but Glycogen phosphorylase b solution exchanged for enzyme dilution buffer. 50ul of G-1-P solution was added to its labeled test tube and incub...

• Word Count: 2070
• Approx Pages: 8
• Grade Level: Undergraduate

... cuvette. The spectrometer was calibrated with the blank cuvette. Then I prepared a mixture of 35 ml of starch and 35 ml of distilled water in a flask. I placed this solution into a water bath to achieve 70°C. Then 11 cuvettes were each filled with 0.1 ml of I2KI . Next 5 ml of the 70 ml solution was measured into one of the 11 cuvettes and ... cuvette. The spectrometer was calibrated with the blank cuvette. Then I prepared a mixture of 35 ml of starch and 35 ml of distilled water in a flask. I placed this solution into a water bath to achieve 70°C. Then 11 cuvettes were each filled wi...

• Word Count: 1671
• Approx Pages: 7
• Has Bibliography
• Grade Level: High School

... Measuring cylinder • Boiling tube (x2) • Rubber tube • Beaker • Bung • Hydrogen peroxide of 20volIce (approx. 20 cubes) • Ruler • Burette • Cork borer • A water bath • Beaker • Ruler Diagram: Set apparatus as shown in the diagram. Cut out a piece of potato using the 10mm cork borer, then cut to a length of 4cm long and ... my prediction. I think the reason for this was because I: used a wide range of results, found the average results which cancelled out anomalies and used accurate measuring facilities, such as; water bath which made us to be able to adjust the tem...

• Word Count: 2395
• Approx Pages: 10
• Grade Level: High School

... test tube was recorded. The same steps were then repeated with the test tube at 35°C, and 65°C respectively. The 35°C, and 65°C test tubes were placed into a 50° hot bath, until optimal temperature was reached. Times were recorded for each test tube using a timer. After testing for the effects of temperature of the substrate on enzymes, the effect of substrate ... ...

• Word Count: 2220
• Approx Pages: 9
• Has Bibliography

... of a large beaker was filled with water, placed on to a hot plate and then set to high. Then a test tube of Catalase and water was placed in the hot water bath after the desired temperature was reached of 90 degrees Celsius. This step was repeated for two more stations with hot plates except the hot plates were set to 4 and 2 after ... ...

• Word Count: 2123
• Approx Pages: 8
• Has Bibliography
• Grade Level: High School

... inefficient heating techniques on the experimenters behalf. Also though, mixing the tubes every minute is inefficient in the sense that you have to remove the test tube from the water bath (heating technique) causing a break in what is supposed to an uninterrupted heating process. In the future another method of keeping the contents well mixed could be used. Each enzyme and ... ...

• Word Count: 1327
• Approx Pages: 5
• Has Bibliography
• Grade Level: High School

... a different pH level (66 data entries). The effects of temperature: Five different water baths were prepared at the following temperatures: 15, 30, 45, 55, 60, and 70 deg C. Each water bath had a separate reaction flask containing 35 ml of starch solution and 35 ml of distilled water. When reaction flasks reached the appropriate temperature the enzyme was added. The basic procedure ... ...

• Word Count: 1676
• Approx Pages: 7
• Grade Level: High School