He is originally introduced in Act 1, scene 1 when Leonato states that he is relieved that Don John has realigned himself with his brother, Don Pedro. Don John states that "I am not a man of many words," leading the audience to come to the conclusion that he is shy. This is quickly proven not to be true when he states how much he hates his brother in Act 1, scene 3, he "would rather be a canker in a hedge than a rose in his grace," This shows that he would rather be something heartless and diminishing than unite with his brother. At this point in the play, the audience is getting a vague idea of who the main antagonists and protagonists are.
There are various parts in the play in which Tybalt and Don John act in very similar ways. For example during a masked ball in "Romeo and Juliet," Tybalt sees Romeo at the Capulet's ball, and therefore instantly wants to kill Romeo, and tells a servant to "fetch me my rapier, boy." Proving that Tybalt will always resort to violence when encountering a Montague, Tybalt's hatred for Montagues is proven to be great, as he knew a Montague was at the party by Romeo's voice. Tybalt then refers Romeo to "a slave," meaning the lower class citizen, just like the rest of the Montagues. He also thinks the Montagues are at the masked ball to "fleer and scorn at our solemnity," meaning that Tybalt feels that the Montagues have come to the party to mock the Capulets. During this scene, the audient is unnerved by their presence as anything could happen. Tybalt is a Catholic character, and as he states "to strike him dead, I hold it not a sin," he is saying that to murder Romeo would not be a sin, as they have hated each other for so long that Tybalt thinks that God would exonerate him for killing Romeo. It also conveys the level of hate and proves that Tybalt is willing to risk eternal damnation to kill Romeo. However, at the masked party in "Much ado," Don John attempts to convince Claudio that the Prince has been fooling with Claudio, just to let him down and get Hero for himself and states that the Prince "woos for himself.