5. Flame sterilize a teasing needle, cool, then pierce skin of disinfected fruit.
6. Unscrew cap on Penicilium italicum culture tube with one hand and flame the mouth of the tube.
7. Using the first three fingers of your writing hand, obtain a sterile applicator stick and remove a small sample of the fungus and smear over the puncture wound of the fruit.
8. Flame tube and recap. Discard swabs appropriately.
9. Obtain a second sample with a sterile applicator stick and smear over an unpunctured section of the fruit.
10. Flame tube and recap. Discard swabs appropriately.
11. Maintain a control for the experiment.
12. Make observations periodically (make note of the fungus" odor, shrinkage, discoloration or growth characteristics).
13.Make a slide of your inoculating organism.
14. Disinfect all work surfaces.
15. Partially unseal bag to allow quick but aseptic entrance for sample collection. .
16. With sterile applicator stick collect a sample of spores (blue-green, moldy decay) from the infected fruit.
17. Streak a Potato Dextrose Agar plate and incubate at room temperature for approximately 5 days.
18. Observe growth of culture periodically with lid intact.
19. Growth may appear as circular, a white fuzzy mass of mycelium or white specks that gradually change color, from white to blue-green. Observe with a steromicroscope.
20. Repeat cleaning and washing procedure as in steps 1-4 with appropriate number and type of fruits.
21. Reinoculate your fruits (hosts) as in steps 5-11.
22. Stain a sample of the organism that you have reisolated and compare with starting culture.
23. Observe and record periodically.
24. Repeat procedures as in steps 14-19.
25. Observe periodically.
26. At the end of the experiment, again stain your organism.
Results.
Macro-.
Several weeks after initial inoculation, a substantial growth was evident. The fungal growth had a distinct odor of mold. The same amount of growth was evident around the puncture-swabbed area as the unpunctured-swabbed area.
Ed Koch and Russel Feingold both present valid arguments both for and against capital punishment. Though both writers have broken down their arguments thoroughly I remain sided with Koch. I feel capital punishment is absolutely necessary and Koch's argument affirmed my theory. ... Ed Koch's argument is much more sensible, logical, and provides comfort to victims. ... I agree with Koch completely that capital punishment does exactly the opposite. ...
Koch is challenging the reader to make a choice. ... Koch has a very strong pro- opinion on the death penalty. I believe that Koch devotes most of his essay to refuting opposition arguments because of deep moral and ethical opinions. Every argument leads back to an inept case made against the death penalty according to Koch. ... Koch's essay in the argument pattern he chose to write in really drove his point home to the reader. ...
Koch is the author of an essay titled "Death and Justice." ... Not only are Koch's arguments weak, but they are also unintelligent. ... Koch also argues that the death penalty is not barbaric. ... Koch goes on to say, "So it's not the method that really troubles opponents. ... Edward Koch has finally seen the light! ...
Two individuals that stand on opposite sides of the debate are Edward Koch and David Bruck. ... Killing is wrong" (Koch 33). ... I hope you have the courage and moral strength to stop the killing" (Koch 33). ... As a district leader, councilman, congressman, and mayor" (Koch 33). ... Koch's argument about the death penalty is effective because of his use of logic. ...
Koch he argues in favor of the death penalty. ... Capital punishment cheapens the value of human life, Koch declares in his next argument. ... Koch argues, justice requires that the law be applied equally to all. Koch also brings the bible into this essay. ... I agree with Edward Koch and all of his arguments in this essay. ...
By Koch being the mayor of New York City when this was written, he already has an established base of ethos. ... Yet, contrary to usual political gibberish that pleases everyone, Koch takes a side... Koch proceeds to emphasize the fact that no other countries have a murder rate close to the United States. ... Once again, Koch appeals to emotions of the readers by using a real-life example. ... Throughout the essay, Koch maintains a very serious and almost dark tone. ...
Koch, is trying to persuade its audience that death penalty is justice. ... This is an example of extrinsic ethos, which gives more power to the authority of Koch. ... First of all, Koch mentions that some people think that the death penalty is "barbaric." ... But Koch also says that that this analogy is imperfect because murder is not the disease. ... In this argument Koch wants to persuade his audience in terms of logic. ...
Edward Koch is someone that would agree that the death penalty brings justice. ... One of the things that Edward koch said in his essay that I found to be true is when he said in his essay that " Human life deserves speacial protection, and one of the best ways to guarantee that protection is to assure that convicted murderers do not kill again. ... Koch, 240). ... Koch, 241). ... Koch, 241). ...