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Cloning

 

            Cloning is a phenomenon commonly referred to today. Right from 1997 when "Dolly" the sheep Dolly was revealed to the world as the 1st mammal to be cloned from the cells of an adult animal. Man has been curious, perhaps disbelieving, fascinated yet apprehensive of the fact that living beings can be created in this manner. "Cloning" is in the actual sense, an umbrella term which refers to "different processes for duplicating biological material.
             The layman worldwide understands cloning as merely "Reproductive cloning" which, in their opinion implied creating life replicas of the parents. However this is a rather incomplete or an incorrect opinion. There are in fact three types of cloning technologies, namely, .
            
             • DNA cloning .
            
             • Therapeutic cloning.
            
             • Reproductive cloning.
             DNA cloning is actually "Recombinant" DNA technology and alternately referred to as "Molecular" cloning or "Gene" cloning. It refers to a transfer of DNA fragments of interest from one organism to a self replicating genetic element. For example: A bacterial plasmid.
             Reproductive cloning on the other hand is a technology used to generate an organism that has the same nuclear DNA as an already existing or previously existing organism. .
             Finally the Therapeutic cloning refers to the developing cells through nuclear transfer for clinical trails in patients who have irreversible brain damage, diabetes, liver ailments, etc.
             As early ass 1952, scientists first demonstrated that they can remove the nucleus from frog egg, replace it with an embryonic frog cell, and then allow the egg to develop into a tadpole. This "Nuclear transfer" transplants an animal gene into an egg. The tadpole is therefore a clone of the embryo that donates its nucleus.
             In 1975 scientists got tadpoles after transferring cell nuclei from adult frogs. Over a decade later, in 1986, a sheep was cloned by nuclear transfer of embryonic cells.


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