1. Identification of Two Unknown Bacteria
... on NA plates at 25∘C (Leboffe & Pierce, 2010). Gram staining was the method used to determine if Unknown 9 had been successfully split into two isolated bacteria. First, a drop of DI water was placed on a slide. An inoculation loop was sterilized, cooled, and used to pick up an isolated colony from a streak plate growing Unknown 9. The loop was gently swirled in the ... drop of water, allowing some of the bacteria on the loop to become suspended in the droplet. Good indication of bacterial concentration is a droplet that is slightly opaque in appearance. The loop was then sterili...
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